| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.071929 |
| Chromosome: | chromosome 3 |
| Location: | 977275 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148050 | POLI1 | DNA polymerase iota; (1 of 1) K03510 - DNA polymerase iota (POLI) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGAGCGGGTGCGCGGCTGGGCGTGGAG |
| Internal bar code: | ACGCGCGGTGGGGCACATAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 99.4387 |
| LEAP-Seq n confirming: | 1063 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCACCACTCGTTTAGT |
| Suggested primer 2: | GTCGTGGAGATCTACGGAGC |