| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071943 |
| Chromosome: | chromosome 2 |
| Location: | 5794171 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110800 | NRT2F | Nitrate/nitrite transporter; (1 of 4) K02575 - MFS transporter, NNP family, nitrate/nitrite transporter (NRT, narK, nrtP, nasA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGTGTGTATGTGTTTTGTGTGTAAGG |
| Internal bar code: | ATTCCCGGCCAATAGCAGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 95.4286 |
| LEAP-Seq n confirming: | 167 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCGTGCATGGAAGGATCT |
| Suggested primer 2: | TTGCATCTGCGTTTACTTGC |