| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071983 |
| Chromosome: | chromosome 3 |
| Location: | 361714 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144807 | MAS1 | (1 of 1) 2.3.3.9 - Malate synthase / Malic-condensing enzyme; Malate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCCCAGCTTTTCGTCGCGACGCTGCAC |
| Internal bar code: | GCTTCTGCTGTAACGCAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 292 |
| LEAP-Seq percent confirming: | 99.0654 |
| LEAP-Seq n confirming: | 742 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAACACAGAGTACCCGCC |
| Suggested primer 2: | CCTGACTCTAACGCGACACA |