| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072035 |
| Chromosome: | chromosome 2 |
| Location: | 2822487 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094551 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGATGGTAGGCGTCTGCCAAACACTGT |
| Internal bar code: | TCGGGACTATGGCAAACAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2349 |
| LEAP-Seq percent confirming: | 99.3413 |
| LEAP-Seq n confirming: | 3318 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTATCGTTCCGTGTTGG |
| Suggested primer 2: | GAGGCTGCACCTACCCATAG |