Insertion junction: LMJ.RY0402.072094_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.072094
Chromosome:chromosome 12
Location:5207980
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g527918 (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACACAAGTCCATGGAAGTGGGCGTCAGC
Internal bar code:TTTGTGTCCGTTCCTAAGCCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:886
LEAP-Seq percent confirming:98.7074
LEAP-Seq n confirming:2520
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGAATCAAGCGGGGAGA
Suggested primer 2:AAATACGTGTGCGACCATCA