| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072099 |
| Chromosome: | chromosome 11 |
| Location: | 1588445 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467760 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGCTTGAGCGGGCCCGCGGGCAGTGGG |
| Internal bar code: | CACTTCACACGATTAACTACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 99.2994 |
| LEAP-Seq n confirming: | 4252 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGAGCTTGACGGGATTG |
| Suggested primer 2: | TATTACTTGGCGACAAGGGC |