| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072123 |
| Chromosome: | chromosome 17 |
| Location: | 2393908 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715250 | BCCP1,BCC1 | (1 of 1) K02160 - acetyl-CoA carboxylase biotin carboxyl carrier protein (accB, bccP); Acetyl-CoA biotin carboxyl carrier | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCGGTCTTTGCCAGCCTTTTGACCGCC |
| Internal bar code: | CCACCATTGGTGACTAGTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1039 |
| LEAP-Seq percent confirming: | 99.7894 |
| LEAP-Seq n confirming: | 7582 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGCTTGCTCTTCAGCTC |
| Suggested primer 2: | AAGGCAGGTGTGGAACAAAC |