| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072132 |
| Chromosome: | chromosome 12 |
| Location: | 3114895 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499950 | CPL18 | (1 of 2) PF07819 - PGAP1-like protein (PGAP1); possible serine esterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCACCAGCACCAGAAGGTGGTCGGGC |
| Internal bar code: | GCCGGTCATCACCGAGCAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 22 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACACGTCACACACCACA |
| Suggested primer 2: | CCTGACTGGGAAGACACGAT |