| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072150 |
| Chromosome: | chromosome_13 |
| Location: | 2379834 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g579450 | CST1 | Chlamy-specific membrane transporter of unknown function | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCGCTCCCCCCTCCGTTGCCTTCCGCAGT |
| Internal bar code: | GCGGAAGGCAAAAGGCGGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 99.0847 |
| LEAP-Seq n confirming: | 1299 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCTCGCTGTTGACTTTG |
| Suggested primer 2: | TGATCAGGAAGCAGTTGACG |