Insertion junction: LMJ.RY0402.072153_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.072153
Chromosome:chromosome 7
Location:6387879
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g357750 (1 of 3) PTHR10383 - SERINE INCORPORATOR intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGTCTGACAATGTGGCTGCGGGGCCATG
Internal bar code:ACGCATAGAGCTTCCCACTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:37
LEAP-Seq percent confirming:53.9295
LEAP-Seq n confirming:199
LEAP-Seq n nonconfirming:170
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCGTATCCACAAGCCATT
Suggested primer 2:CTTTTGCAAGTAGCTTGGGC