Insertion junction: LMJ.RY0402.072161_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.072161
Chromosome:chromosome 9
Location:2597914
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g390450 POB15 Proteome of basal body 15; (1 of 88) IPR011333 - POZ domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGACTTCGCGGACTGCCGAATCGTGTT
Internal bar code:TGGAGGAATAGTCAAAGGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:59
LEAP-Seq percent confirming:96.267
LEAP-Seq n confirming:851
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTTAGGTACCCGGCTTGTGA
Suggested primer 2:ACACACACACACACACACCG