| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072166 |
| Chromosome: | chromosome 11 |
| Location: | 435440 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467587 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATCTTGATGAGTACGGTAAACTGCTCA |
| Internal bar code: | TCATTACGTGGGTCTAGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 99.0156 |
| LEAP-Seq n confirming: | 4627 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGACCTGCTTGTGTCAGC |
| Suggested primer 2: | AGTGTTGAATAAGGGCGGTG |