| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072185 |
| Chromosome: | chromosome 9 |
| Location: | 6980338 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410750 | NIR1,NII1 | Nitrite reductase, chloroplastic; (1 of 1) 1.7.7.1 - Ferredoxin--nitrite reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTCTGGGCCCCTTGCCCCCTCTCCCTC |
| Internal bar code: | GATCCTTTACATGGACGACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 35.0772 |
| LEAP-Seq n confirming: | 2045 |
| LEAP-Seq n nonconfirming: | 3785 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCACCACGTGCATATC |
| Suggested primer 2: | TTTCCCGTCAGCTCTGTCTT |