Insertion junction: LMJ.RY0402.072220_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.072220
Chromosome:chromosome 1
Location:1031174
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g005950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTTCGTACTGCTCGCTAACCGGATTTCA
Internal bar code:CCATGAAGGGCACGAAGACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:206
LEAP-Seq percent confirming:50.3181
LEAP-Seq n confirming:2768
LEAP-Seq n nonconfirming:2733
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATTACGACTCCCACACCTGC
Suggested primer 2:AGGTGAAGGTTGTGAGGTGG