Insertion junction: LMJ.RY0402.072227_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.072227
Chromosome:chromosome 3
Location:5589322
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g186750 (1 of 7) IPR011989//IPR016024 - Armadillo-like helical // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGCTGCTCGCCAACGCCATGAGCCACCC
Internal bar code:CTTTCGCGCGACGAGTCACCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:210
LEAP-Seq percent confirming:97.5013
LEAP-Seq n confirming:1834
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACACATTCAGTGCACACGC
Suggested primer 2:AGGTCTGGGTTAGGCCACTT