Insertion junction: LMJ.RY0402.072246_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.072246
Chromosome:chromosome 2
Location:4026942
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g097200 SRR14 Scavenger receptor cysteine rich (SRCR) protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGGGGAGAACAGTCCTCTTCCGCTTCC
Internal bar code:AGTAGGCCATGGTCGGATGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:51
LEAP-Seq percent confirming:25.7951
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:210
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACTGTGGGTGGACAGTG
Suggested primer 2:GAGCCCACTGTCTTTCTTGC