| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072266 |
| Chromosome: | chromosome 4 |
| Location: | 2218642 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220000 | (1 of 4) IPR000719//IPR001245//IPR002290//IPR011009 - Protein kinase domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATGGTGGTGTTGAGTGTGCTTCCGGTT |
| Internal bar code: | CTGCTTGCTTCTACGTTAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3 |
| LEAP-Seq percent confirming: | 89.9061 |
| LEAP-Seq n confirming: | 1149 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAGCTAGAGCTGGGGAG |
| Suggested primer 2: | GGGTTCGCTGCTTTCTAGTG |