Insertion junction: LMJ.RY0402.072274_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.072274
Chromosome:chromosome 3
Location:4881687
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g179350 MFT16 Major facilitator superfamily transporter; (1 of 1) PF03825//PF07690 - Nucleoside H+ symporter (Nuc_H_symport) // Major Facilitator Superfamily (MFS_1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCGCCAAAGCCTACGCACGCAAAGCGG
Internal bar code:GAATGTCGATAATCTCATTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:573
LEAP-Seq percent confirming:99.5904
LEAP-Seq n confirming:7537
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATACCTGGTTGACCGACAGC
Suggested primer 2:ATGGCTTCCGTGACACCTAC