| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072287 |
| Chromosome: | chromosome 3 |
| Location: | 1637464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152950 | PUS12 | (1 of 1) 4.2.1.70//5.4.99.12 - Pseudouridylate synthase / Uracil hydrolyase // tRNA pseudouridine(38-40) synthase / tRNA pseudouridylate synthase I; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGTGGAAGGCGTCCATGTATCAGGTG |
| Internal bar code: | TAGGTTTAAGTGTTCTGTACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 0.8 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 248 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCATCGTCCTCCTCATC |
| Suggested primer 2: | GCGTAGGACAGTGTATGCGA |