Insertion junction: LMJ.RY0402.072291_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.072291
Chromosome:chromosome 13
Location:2798838
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g582900 (1 of 77) IPR001623 - DnaJ domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCCTCGGCACCCTTACCACTTGAGAGCT
Internal bar code:CGTCTGCACGCGTGCGAGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1112
LEAP-Seq percent confirming:99.5232
LEAP-Seq n confirming:835
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TAAACTTGGCCCAGTCAACC
Suggested primer 2:CTCACAGGAACACCATGTCG