| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072299 |
| Chromosome: | chromosome 9 |
| Location: | 1865168 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395600 | OBGC,OBGC1 | Factor possibly involved in assembly of the ribosomal 50S subunit; (1 of 1) PTHR11702:SF3 - GTP-BINDING PROTEIN 10 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCATCAGAGGAATTGAGATGCTAAGCGG |
| Internal bar code: | CACCTTGACGGGTCTAGCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 99.8325 |
| LEAP-Seq n confirming: | 596 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTAACCCTACCCTGAA |
| Suggested primer 2: | ACACCTGTTTCCCAACAAGC |