| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072315 |
| Chromosome: | chromosome 3 |
| Location: | 8360891 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198750 | (1 of 2) K02327 - DNA polymerase delta subunit 1 (POLD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTACCCACCAGTTCGGCTTAGTTTTGGC |
| Internal bar code: | CATAGCTGTCGTAGGAGACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6 |
| LEAP-Seq percent confirming: | 99.7352 |
| LEAP-Seq n confirming: | 5649 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGTGGTTCAGTTGATGG |
| Suggested primer 2: | TGTACCAGGGTGGTGTAGCA |