Insertion junction: LMJ.RY0402.072324_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.072324
Chromosome:chromosome 17
Location:2693836
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g717900 PHC1 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTAGTCTGCGTCATTGCATATTTTGTCA
Internal bar code:TAACATAGGCCTCTGCCGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:811
LEAP-Seq percent confirming:97.7679
LEAP-Seq n confirming:219
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACCACACATCCACACGAGA
Suggested primer 2:CAGTCGTTCTGGCTGTCAAA