| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072325 |
| Chromosome: | chromosome 12 |
| Location: | 1207987 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489600 | (1 of 3) IPR003029//IPR012340//IPR022967 - S1 domain // Nucleic acid-binding, OB-fold // RNA-binding domain, S1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCGCTGCCGGGGCCGCCGCCCATGCC |
| Internal bar code: | GCCCCGACCCAGGCGCCCTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 75.764 |
| LEAP-Seq n confirming: | 1066 |
| LEAP-Seq n nonconfirming: | 341 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCCACCGTTAAAATCCT |
| Suggested primer 2: | CCTCCTCCTCGCTATCACTG |