| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072360 |
| Chromosome: | chromosome 12 |
| Location: | 2137977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508852 | (1 of 1) 6.3.4.10 - Biotin--[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase / Propionyl-CoA holocarboxylase synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCGCGGGAGTTCCTGGCCCCAGGCGT |
| Internal bar code: | GTCACGCCTACCCGCTGCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.7447 |
| LEAP-Seq n confirming: | 3125 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATGGGGTTGGAGGTTTT |
| Suggested primer 2: | GCAACTTTGCCCGTATTCAT |