| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072399 |
| Chromosome: | chromosome 2 |
| Location: | 5619684 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109250 | (1 of 1) PF13394 - 4Fe-4S single cluster domain (Fer4_14) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGAGCGGCTGCTGGCGCTGACCTCCGA |
| Internal bar code: | AACGGGCGCCTTCCGGCGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 98.1946 |
| LEAP-Seq n confirming: | 979 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGGAGAAGGGTGGAAC |
| Suggested primer 2: | ATATGACAGACTCCCACGGC |