Insertion junction: LMJ.RY0402.072417_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.072417
Chromosome:chromosome 13
Location:3437659
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g587400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACTCAGAGCACAACCTGACAAGACTTCA
Internal bar code:CGCGAGTAATCTTTGTGATTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:931
LEAP-Seq percent confirming:99.0013
LEAP-Seq n confirming:793
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGTTTGAGCTGCTCGTT
Suggested primer 2:TGGGAATGTGTGATTGTGCT