| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072431 |
| Chromosome: | chromosome_1 |
| Location: | 1753494 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009400 | NSS2 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGTCATGGCGTTGGGCCAAATGATTTGGT |
| Internal bar code: | CGTTTGGTGCAGTAGTCTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.1993 |
| LEAP-Seq n confirming: | 26636 |
| LEAP-Seq n nonconfirming: | 215 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGCTGCTGGAAATGAAT |
| Suggested primer 2: | GAATGCACCTACCCTCCAAA |