Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.072431 |
Chromosome: | chromosome 1 |
Location: | 1753494 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g009400 | NSS2 | (1 of 2) PTHR11819//PTHR11819:SF12 - SODIUM/SOLUTE SYMPORTER // HIGH-AFFINITY CHOLINE TRANSPORTER 1; Sodium:solute symporter | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCATGGCGTTGGGCCAAATGATTTGGT |
Internal bar code: | CGTTTGGTGCAGTAGTCTGCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 417 |
LEAP-Seq percent confirming: | 99.1993 |
LEAP-Seq n confirming: | 26636 |
LEAP-Seq n nonconfirming: | 215 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGGCTGCTGGAAATGAAT |
Suggested primer 2: | GAATGCACCTACCCTCCAAA |