| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072431 |
| Chromosome: | chromosome 14 |
| Location: | 3215722 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629150 | CYG4 | Adenylate/guanylate cyclase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGCCAAGGCCGCTTCCAGCAGCCGCGA |
| Internal bar code: | CAAATGCTACCCCGCAGCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 15.1221 |
| LEAP-Seq n confirming: | 576 |
| LEAP-Seq n nonconfirming: | 3233 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCAGTCGTGTCGTCAT |
| Suggested primer 2: | CCTGGTCACTTTAAGCCTGC |