| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072467 |
| Chromosome: | chromosome 2 |
| Location: | 8389719 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141546 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCACCGCTTCAGCCGACGCCGCGTTGGG |
| Internal bar code: | TCCGCGGCCTACAACCCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 80.6916 |
| LEAP-Seq n confirming: | 280 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCCTCAAAACTCAGTAGC |
| Suggested primer 2: | TGGCACTTTGTTTCCTACCC |