| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072489 |
| Chromosome: | chromosome 6 |
| Location: | 3158400 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275450 | (1 of 1) PF00069//PF13855 - Protein kinase domain (Pkinase) // Leucine rich repeat (LRR_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACATCACGACCCCTCGCGCCCTCGCCTC |
| Internal bar code: | CCGGCAGGTAGGCGCACCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 74.778 |
| LEAP-Seq n confirming: | 4631 |
| LEAP-Seq n nonconfirming: | 1562 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGAGATGCAGGTGACT |
| Suggested primer 2: | GCTACTGCTTCGGAGGAATG |