| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072512 |
| Chromosome: | chromosome 2 |
| Location: | 6822688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119100 | CER2 | (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME); Carboxyesterase-related protein/arylformamidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATCGCCCAAGGATCCGACAAGCACGGC |
| Internal bar code: | GTTCCCAATGCTGACCCTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGTAGCGTCACTATGC |
| Suggested primer 2: | CGAGTGACACAAGCTCCTCA |