| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072521 |
| Chromosome: | chromosome 10 |
| Location: | 4941098 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455000 | (1 of 1) IPR010995//IPR011009 - DNA repair Rad51/transcription factor NusA, alpha-helical // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCAGTGGGGCTGCGGTGCCAATCTACAT |
| Internal bar code: | CTATGAAAAGAGGCGGTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 57.9936 |
| LEAP-Seq n confirming: | 1266 |
| LEAP-Seq n nonconfirming: | 917 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATGCATCCTTAGTCCGC |
| Suggested primer 2: | GCAGGACGTGTACAGCAAGA |