Insertion junction: LMJ.RY0402.072553_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.072553
Chromosome:chromosome 7
Location:4415313
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g340850 CYP743A2,CYP19 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGCACAAGCAGAGGTGTTGCTGTGCATA
Internal bar code:TGTCTTGCGGAGATCGGGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:550
LEAP-Seq percent confirming:99.7651
LEAP-Seq n confirming:3822
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TCATCGAAAACGCAACCATA
Suggested primer 2:GCCAAAAGACACCACCTTGT