| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072554 |
| Chromosome: | chromosome 8 |
| Location: | 3886176 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378000 | (1 of 1) K18749 - protein LSM14 (LSM14, RAP55, SCD6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGAGCCGTGCTCATCAAGGAAAAGGTT |
| Internal bar code: | GTGGAGTCGATCCCGGGATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 99.754 |
| LEAP-Seq n confirming: | 811 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACTCGCCTATCAGCCTT |
| Suggested primer 2: | ACATAATCGCTTACCAGCGG |