| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072559 |
| Chromosome: | chromosome 3 |
| Location: | 3589089 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168350 | (1 of 1) K06947 - polynucleotide 5'-hydroxyl-kinase GRC3/NOL9 [EC:2.7.1.-] (GRC3, NOL9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGTGTGAGCTTCGTGTAAACTCAGAGA |
| Internal bar code: | GTGCGGATGGATGTCATTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 99.8031 |
| LEAP-Seq n confirming: | 7603 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAAGATGACGCAGAACA |
| Suggested primer 2: | ACAACCCTCGTTCCTTCCTT |