| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072597 |
| Chromosome: | chromosome 9 |
| Location: | 6889264 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410150 | (1 of 140) IPR002048 - EF-hand domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACGCCGTCATCCAACAGGCGCCGGCCC |
| Internal bar code: | TTCCCGTGGGCACACGTGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 67.2796 |
| LEAP-Seq n confirming: | 1244 |
| LEAP-Seq n nonconfirming: | 605 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTGTTAGGCTCGAATCC |
| Suggested primer 2: | GCAACCTGGTGGTGAGAGAT |