| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072604 |
| Chromosome: | chromosome 1 |
| Location: | 7013048 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050616 | 3'UTR | ||
| Cre01.g050624 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGTTTAAGGACGCCTTCGCCTGAAGGT |
| Internal bar code: | GCATACGGTGTGCGCGCCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 99.7952 |
| LEAP-Seq n confirming: | 1949 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGTATGCATGCTGCTGGC |
| Suggested primer 2: | TGCTAGGCGAGGTTCTGTTT |