| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072609 |
| Chromosome: | chromosome 16 |
| Location: | 2347727 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659650 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTCATGTGTCGCACGCTTGACACCGCA |
| Internal bar code: | GGCAGAGGCCATCATTTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAACCAACAGCAGTAGCA |
| Suggested primer 2: | CTTACTTCCGCAATTGGCAT |