| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072610 |
| Chromosome: | chromosome 12 |
| Location: | 2503135 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506500 | HTR35 | Histone H3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTCGACGGCGACACGAGAGCAGACGAGT |
| Internal bar code: | TAGACACCGTGTTCGCACGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 98.9362 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGAGGACACCAACCTGT |
| Suggested primer 2: | AGCATGACAAGAGGCGCTAT |