| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072617 |
| Chromosome: | chromosome 9 |
| Location: | 2259930 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392652 | (1 of 1) 1.5.1.22 - Strombine dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGGGGCGCACACGTAAGCCCACGAGC |
| Internal bar code: | CCAGACAACTACATTGTGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 72.6962 |
| LEAP-Seq n confirming: | 213 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATCAGTAGGGCTTTCGC |
| Suggested primer 2: | ACCATGGATAAAGTGCTCGG |