| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072623 |
| Chromosome: | chromosome_7 |
| Location: | 3991163 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g339850 | antisense | CDS|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGGTGAGGCGTAGGCTGCAGGTCGGCGCT |
| Internal bar code: | GGGCCGGGCTGGTCCCGGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 83.1966 |
| LEAP-Seq n confirming: | 4258 |
| LEAP-Seq n nonconfirming: | 860 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGCAGACTTCGCACCAG |
| Suggested primer 2: | GGCCCTTTTGCTCAATGATA |