| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072644 |
| Chromosome: | chromosome 1 |
| Location: | 5853374 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041800 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGTGAGGCTGTAGCAAGCGTTCCCCCA |
| Internal bar code: | ACGAAGGCTTCCCCGCTGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 75.4167 |
| LEAP-Seq n confirming: | 181 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAGGGTAGCCCTCCTCC |
| Suggested primer 2: | AAAATCCTCAAACACGCTCG |