| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072646 |
| Chromosome: | chromosome 17 |
| Location: | 4441823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732050 | AGE3 | DNA repair glycosylase; (1 of 1) K10773 - endonuclease III (NTH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCTACCCTGGCTCTGACACTCGGATGCC |
| Internal bar code: | ATGTGGAGTGGGTCGGTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 79.8651 |
| LEAP-Seq n confirming: | 2487 |
| LEAP-Seq n nonconfirming: | 627 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTTTCAGCTGTTGG |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |