| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072667 |
| Chromosome: | chromosome 12 |
| Location: | 3307998 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATTCAACTATGGGTGACGACGGTCCAC |
| Internal bar code: | AATGCAGGTGAGTGAGGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 99.5902 |
| LEAP-Seq n confirming: | 729 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGAACACCCGGACAGTT |
| Suggested primer 2: | GTCACTGCTGCTGCTACTGC |