| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072704 |
| Chromosome: | chromosome 12 |
| Location: | 7186685 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558550 | CPLD60 | (1 of 1) PTHR10992:SF351 - HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN; possible hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCGGCGAGGAAGGGGGCACGCCAGGGC |
| Internal bar code: | CGATCGCGCCAGTGAATCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 68.1275 |
| LEAP-Seq n confirming: | 855 |
| LEAP-Seq n nonconfirming: | 400 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGGTGTTGTGCAATGTG |
| Suggested primer 2: | AGGTATACAGCATCCGACCG |