| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072719 |
| Chromosome: | chromosome 16 |
| Location: | 4363997 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669525 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGATCGTTCTTCACATGAAGGGCTAC |
| Internal bar code: | GGTCTGGAGGGGTTATTCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 28 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTGTCTGTGGGTG |
| Suggested primer 2: | GTTGTGTGAGGTTTGGGCTT |