Insertion junction: LMJ.RY0402.072721_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.072721
Chromosome:chromosome 9
Location:6199180
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g406000 BPL1 Biotin-protein ligase; (1 of 1) 6.3.4.11//6.3.4.15 - Biotin--[methylcrotonoyl-CoA-carboxylase] ligase / Biotin--[methylcrotonoyl-CoA-carboxylase] synthetase // Biotin--[acetyl-CoA-carboxylase] ligase / Biotin--protein ligase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCCCTATCCTGGTACCATGCAGCCCCT
Internal bar code:GTGTGTGAAGTCAAGGCCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:183
LEAP-Seq percent confirming:99.4105
LEAP-Seq n confirming:1349
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACACAATACCGCTGTC
Suggested primer 2:TTGATGTTTCGCGTGCTAAG