Insertion junction: LMJ.RY0402.072721_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.072721
Chromosome:chromosome 12
Location:2196223
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g508350 TPR2,TPR11 (1 of 26) IPR013026//IPR019734 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat; Tetratricopeptide-repeat protein 11 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAAACGACAGCAGGAACAGGGAGGGAGC
Internal bar code:GTATATTCTGCTCTAGTAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:8
LEAP-Seq percent confirming:99.7739
LEAP-Seq n confirming:1765
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTCACAGAAACCCAACAT
Suggested primer 2:GGAAATGTGTTGGGTCTGCT