| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072721 |
| Chromosome: | chromosome 12 |
| Location: | 7194927 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558526 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGACCCTGCTGCTGTACACACGAACACA |
| Internal bar code: | GGTCCAGGCTTAGTCGAAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 90.0527 |
| LEAP-Seq n confirming: | 1539 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCTTTCCCAAAGTGGTA |
| Suggested primer 2: | TAGCTCATGTCAGCCCACTG |